Evaluation of the larvicidal activity of essential oils from some plants on Culicidae larvae (Diptera; Nematocera)

Author : Lounaci Zohra, Mekliche Dyhia, Belkadi Lynda, Khemmar Saada, Oussad Nadia, Medjdoub Bensaad Ferroudja

Abstract :The fourth instar larvae of Culiseta longeareolata were the subject of a toxicological study using the essential oils of Eucalyptus globulus and Ocimum basilicum. Different doses of 10%, 5%, 2.5%, 1.25% have been conducted in the laboratory on the L4 larval stages. These preliminary tests carried out according to a methodology inspired by the standard World Health Organization protocol revealed the significant evolution of mortality as a function of time and of the doses used for the two bio pesticides. Eucalyptus globulus essential oil has remarkable larvicidal properties; it induces 90% mortality after 72 hours of treatment at a concentration of 10%. The DL50 and DL90 values against fourth instar larvae were 120 and 260 g/l for Cs. longiareolata. The results proved the potential use of this natural product as an alternative to synthetic insecticides for controlling mosquitoes.

Keywords :Culisita longeareolata, formulated essential oils, Eucalyptus globulus, Ocimum basilicum, larva, mortality, toxicity test.

Conference Name :International Conference on Agricultural, Genetics, Biological & Environmental Sciences (ICAGBES-24)

Conference Place Paris, France

Conference Date 17th Dec 2024