Effect of quercetin on spontaneous contraction and peroxyl radicals-induced alterations of spontaneous contraction of longitudinal strips of rabbit ileum

Author : Dalila MESSAOUDI, Kamel GHERZOULI

Abstract :The aim of the present study is to evaluate the effect of quercetin on the spontaneous contraction and free radicals-induced alteration of the rythmic contraction of longitudinal strips of rabbit ileum in vitro. The contractile activity of these longitudinal strips was characterized by a mean tension of 0.84 ± 0.02 g and a frequency of 10.47 ± 0.37 cpm. 30 and 100 μM quercetin decreased the maximal tension of spontaneous contractions by 34.8% and 46.0%, respectively. Peroxyl radicals derived from 2,2` Azobis (2-methylpropionamidine) (ABAP) decomposition led during the first two minutes to an abrupt arrest of the spontaneous contractile contractions of longitudinal strips and a decrease of the basal tone. An increase of 16.5% of the mean tension and a reduction of the frequency of the contractions (from 11.47 ± 0.43 cpm to 6.36 ± 0.39 cpm) was observed after 30 minutes of incubation. Quercetin (100 μM) prevented the disappearance of the spontaneous contractions during the first minutes after the addition of ABAP, and lowered the ABAP-induced tension increase by 30.7%. These results provide a pharmacological basis for the traditional use of quercetin-rich herbal medicines for the relief and treatment of gastrointestinal disorders.

Keywords :Longitudinal strips, Ileum, Quercetin, Contractile activity, peroxyl radicals.

Conference Name :International Conference on Gastroenterology, Digestive System Disorders, Conditions and Treatments (ICGDSDCT-24)

Conference Place Giza, Egypt

Conference Date 17th Dec 2024