Dual Enzyme Inhibition and Antiglycation Activity of the Ayurveda Polyherbal Decoction in Type 2 Diabetes Mellitus

Author : Prof.S.D. Hapuarachchi, KDC Madhushika TS Suresh PK Perera LADM Arawwawala

Abstract :Type 2 diabetes mellitus (T2DM) is commonly managed with herbal formulations in Ayurveda and Traditional Medicine. Balabilvashunti (BBS) Ayurveda Polyherbal decoction, prepared from Sida alnifolia (L.) Ugbor, Aegle marmelos (L.) Corrêa root bark, and Zingiber officinale Roscoe rhizome, is traditionally prescribed for T2DM. This study investigated the antidiabetic potential of BBS through inhibition of carbohydrate-hydrolyzing enzymes and protein glycation. BBS was prepared using the classical decoction method and converted into spray-dried and freeze-dried powders. In-vitro assays were performed to evaluate α-amylase and α-glucosidase inhibitory activity, as well as protein antiglycation, with acarbose and rutin serving as positive controls. Both spray-dried and freeze dried powders showed strong α-amylase inhibition, with IC₅₀ values of 4.54 ± 0.07 mg/mL and 5.01 ± 0.17 mg/mL respectively, compared with acarbose (61.45 ± 2.01 µg/mL). Moreover, for α-glucosidase inhibition, IC₅₀ values were 2.38 ± 0.09 mg/mL (spray dried) and 2.82 ± 0.11 mg/mL (freeze-dried), outperforming acarbose (454.23 ± 1.89 µg/mL). Antiglycation activity was moderate, with IC₅₀ values of 528.36 ± 15.27 µg/mL (spray-dried) and 445.46 ± 8.66 µg/mL (freeze-dried), compared to rutin (316.78 ± 12.43 µg/mL). These results confirm the dual inhibitory activity of BBS on α-amylase and α-glucosidase, supporting its traditional use in T2DM. Although antiglycation effects were less pronounced, the findings highlight BBS as a promising candidate for standardized phytopharmaceutical development in diabetes management

Keywords :Ayurveda, Traditional Medicine, Balabilvashunti, type 2 diabetes mellitus, α-amylase inhibition, α-glucosidase inhibition, antiglycation, phytopharmaceuticals

Conference Name :International Conference on Medicinal Plants & Natural Products Research (ICMPNPR-25)

Conference Place Sydney,Australia

Conference Date 21st Nov 2025