The Effect of Centella Asiatica L. Extract on Callus Formation in the Healing Process of Femur Fracture in White Rats (Rattus norvegicus) as an Animal Model.

Author : Ricko Surya Harahap, Armia Indra Nur Alam, Dasrul

Abstract :Background: Bone fractures pose a risk of complications if healing is disrupted, influenced by both mechanical and metabolic factors. Centella asiatica, rich in glycosides, may accelerate healing by inhibiting pro-inflammatory cytokines. Objective: To evaluate the effect of Centella asiatica on callus activity during femur fracture healing in white rats (Rattus norvegicus). Method: This laboratory experimental study used a post-test only control group design with a complete randomized design (CRD). Thirty Wistar rats (Rattus norvegicus) were divided into three groups: a positive control (fractures treated with NaCl) and two treatment groups receiving Centella asiatica extract at doses of 50 mg/kg BW and 100 mg/kg BW. Observations were conducted on days 7, 14, and 21. Results: This study shows that Centella asiatica L. leaf extract significantly enhances callus formation during femur fracture healing in Wistar rats. Both the 50 mg/KgBW/day (Treatment 1) and 100 mg/KgBW/day (Treatment 2) doses increased callus volume compared to the control group, with significant differences observed (P = 0.0001). The higher dose (Treatment 2) resulted in a larger callus volume, suggesting that higher doses of Centella asiatica L. extract accelerate fracture healing.

Keywords :Bone fracture healing, Centella asiatica, callus formation, Wistar rats, cytokine inhibition, experimental study.

Conference Name :International Conference on Facial Plastic Surgery and Reconstructive Surgery (ICFPSRS-25)

Conference Place Bali, Indonesia

Conference Date 10th Jan 2025