Antibiotic Resistance Profiles Of Aeromonas Veronii From Cultured Fish

Author : Volkan KAYDU1, Merve Gizem SEZENER KABAY, Belgin SIRIKEN1, İrfan EROL, Halil İbrahim ADIGÜZEL

Abstract :A total of 28 Aeromonas (A.) veronii isolates were isolated from 47 cultured fish [gilt-head bream (Sparus aurata), sea bass (Dicentrarchus labrax), and Turkish salmon (Oncorhynchus mykiss)] sold in the Samsun region using the classical culture technique (Violet Red Bile Glucose Agar) and identified with the MALDI-TOF MS system (MS-Matrix-assisted laser desorption/ionization (MALDI) and mass analyzer is time-of-flight (TOF) analyzer). The Kirby-Bauer Disk Diffusion method was used to determine the antibiotic resistance profiles, and evaluated according to the Clinical and Laboratory Standards Institute (CLSI, 2021) guidelines and EUCAST (2023). According to findings, 28 A. veronii isolates showed resistance to nine different antibiotic classes, with resistance rates ranging from 7.14% to 89.28%; 1) Betalactam group antibiotics: Penicillins: ampicillin (AMP; 10 µg) at 85.71%; amoxicillin/clavulanic acid (AMC; 20/10 µg) at 10.71%; ampicillin/sulbactam (SAM; 20 µg) at 89.28%; Cephalosporins: cefepime (FEP; 30 µg) at 7.14%; cefotaxime (CTX; 30 µg) at 7.14%; ceftriaxone (CRO; 30 µg) at 7.14%, ceftazidime (CAZ; 30 µg) at 7.14% and cefoxitin (CFX; 30 µg) at 14.28%; Carbapenems: imipenem (IMP; 10 µg) and meropenem (MEM; 10 µg) both at 10.71%; Monobactams: aztreonam (ATM; 30 µg) at 10.71%; 2) Aminoglycosides, gentamicin (CN; 10 µg) and tobramycin (TOB; 10 µg) both at 7.14%; 3) Tetracyclines: tetracycline (TE; 30 µg) at 17.85%; 4) Quinolones: nalidixic acid (NAL; 30 µg) at 35.71%; 5) Phosphomycin: fosfomycin (FF; 200 µg) at 7.14%; 6) Sulfonamide derivative antibiotics: trimethoprim/sulfamethoxazole (SXT; 1.25/23.75 µg) at 7.14%, while they were found to be sensitive to 7) Amphenicols: chloramphenicol (CHL; 30 µg)]; 8) Glycylcycline: tigecycline (TGC; 15 µg) and 9) Fluoroquinolones: ciprofloxacin (CIP; 5 µg) and levofloxacin (LEV; 5 µg). Addition, of the 28 isolates, 5 were found to be multidrug-resistant (MDR). The MDR findings, it was determined that 27.2% were of Turkish salmon origin and 22.2% of the isolates were of sea bass origin. No MDR isolates were in sea bream origin. In conclusion, A. veronii is one of the widespread pathogenic Aeromonas species in cultured fish and also poses a potential

Keywords :Aeromonas veronii, cultured fish, antibiotic resistance, MALDI-TOF MS, Kirby-Bauer Disk Diffusion, CLSI 2021, EUCAST 2023, betalactam antibiotics, ampicillin, amoxicillin/clavulanic acid, cephalosporins, carbapenems, monobactams, aztreonam, aminoglycosides, gentamicin, tobramycin.

Conference Name :International Conference on Food Science, Food Safety and Food Hygiene (ICFSFSFH -25)

Conference Place Geneva, Switzerland

Conference Date 4th Jan 2025