Evaluation of Anti-sickling activities of three Cissus species; Cissus populnea, Cissus arguta Hook.f. and Cissus quadrangularis L

Author : Timilehin Eunice Oyelakin, Nafisat Kehinde Ogunwale , Solomon A. Ogunmefun, Adeola O. Tejumade, Temitayo O. Ajayi

Abstract :Sickle cell disease (SCD) is an inherited genetic disease that affects people globally, with the highest prevalence in the sub-Saharan African region. Medicinal plants are useful in managing sickle cell crises associated with morbidities. The Cissus family Vitaceae species have been reported to manage sickle cell disease in folklore medicine. Hence, this study aimed to evaluate the antisickling properties of three Cissus species in Nigeria. The leaves and stems of Cissus polpulnea, Cissus arguta, and Cissus quadrangularis were collected, authenticated, dried, pulverized, extracted, and concentrated in vacuo. Antisickling assays followed standard protocols of reversal and inhibition of sickling at 45mins and the samples were also screened for Phytochemicals The Phytochemical screening showed the presence of secondary metabolites except saponins, which were absent in C. quadrangularis leaf. C. polpunea leaf and stem extracts (2 mg/ml) both showed inhibited sickling at 59.6% and reversed at 56.98%. At 4mg/ml, the percent inhibition was 38.02% and 58.6% respectively and both extracts showed no activity for the reversal of sickling. C. arguta leaf and stem extracts (2 mg/ml) showed inhibition at 80.02% and 83.48%, and reversal at 86.98% and 83.48% respectively comparable to the standard Para hydroxybenzoic acid (PABA). At 4

Keywords :Anti-sickling, Sickle cell disease, Cissus arguta, Cissus quadrangularis

Conference Name :International Conference on Natural, Traditional & Alternative Medicine (ICNTAM-25)

Conference Place Toronto, Canada

Conference Date 1st Aug 2025