Expression, Characterization, And Stability Of Milk Exosomal Mirnas And Their Potential As Biomarkers For Sub-Clinical Mastitis In Buffaloes

Author : Korsapati L. Yoshitha, Shailesh D. Ingole, Simin V. Bharucha, Mandakranta Bhuyan, Rajesh R. Pharande, Rajiv V. Gaikwad

Abstract :Milk exosomal microRNAs (miRNAs) are bioactive molecules that regulate cellular communication at the post-transcriptional level. These miRNAs have gained significant attention due to their stability and potential biological roles. This study aimed to evaluate the expression of exosomal miR-148a and miR-186 in normal, sub-clinical mastitis and pasteurized milk to explore their potential as biomarkers for early diagnosis of sub-clinical mastitis. We investigated their stability under extreme boiling conditions, making this the first study to examine miRNA expression and exosome characterization in boiled and pasteurized milk. The size, morphology, and integrity of exosomes were characterized by TEM. A total of 30 milk samples were collected from the Mumbai region, including 20 samples from buffalo farms, categorized into normal (n=10) and sub-clinical mastitis (n=10) groups. Additionally, 10

Keywords :miRNA, sub-clinical mastitis, buffaloes.

Conference Name :International Conference on Veterinary Physiology and Anatomy (ICVPA-25)

Conference Place Lisbon, Portugal

Conference Date 28th Jun 2025