A Comprehensive Preclinical Model of Postmenopausal Cardiometabolic Dysfunction: Combining Estrogen Depletion, Obesity, and Diabetes in Female Wistar Rats

Author : Tanya Densil, Safaa Fathima , Raya Famin Harid , Shakta Mani Satyam , Abdul

Abstract :Background: Cardiovascular diseases represent the primary cause of mortality in postmenopausal women, largely attributable to metabolic conditions such as obesity and diabetes mellitus. The absence of a robust preclinical model that incorporates these risk factors impedes progress in cardiovascular research. This study sought to establish and validate a multifactorial preclinical cardiometabolic disease model in 12 female Wistar rats, integrating ovariectomy-induced estrogen depletion, high fat diet-induced obesity, and streptozotocin-nicotinamide-induced diabetes mellitus to simulate the cardiometabolic risk profile observed in postmenopausal women. Methods: The model was established in three phases over 25 weeks: (i) Ovariectomy to mimic estrogen deficiency, (ii) High-fat diet administration to induce obesity, and (iii) Diabetes mellitus induction using streptozotocin (50 mg/kg) and nicotinamide (110 mg/kg). Metabolic, cardiovascular, and inflammatory markers were assessed, including fasting blood glucose, lipid profile, electrocardiogram abnormalities, cardiac injury biomarkers (Troponin T, CK-MB), insulin resistance (HOMA-IR), and inflammatory cytokines (IL-6, IL-1β, TNF-α)

Keywords :Cardiometabolic model, postmenopausal women, ovariectomy, obesity, diabetes mellitus Wistar rats, estrogen deficiency, cardiovascular risk, inflammatory biomarkers Ask ChatGPT

Conference Name :International Conference on Diabetes, Endocrine and Metabolic Disorders (ICDEMB-25)

Conference Place Chicago, USA

Conference Date 21st Jun 2025